IDWeek | Karius

Learning Lounge Presentation

Friday, October 4

10:15 – 11:00 a.m.
Learning Lounge 1

 

Diagnosing and Predicting Infections Using the Karius Test

 

Speakers 

  • John Farrell, MD | Director of Microbiology and Serology Labs | OSF System Laboratory
  • Joshua Wolf, MBBS, FRACP | Pediatric Infectious Diseases Physician and Medical Director of Antimicrobial Stewardship | St. Jude Children's Research Hospital
  • Asim Ahmed, MD | Senior Medical Director | Karius

 

Program Description

The Karius® Test is a non-invasive blood test based on next-generation sequencing of microbial cell-free DNA. Physicians will describe the utility of the Karius Test in current clinical practice and describe potential future applications in predicting infections in vulnerable populations.

 

Karius Booth Presentations

Join us at Karius Booth #429 for exclusive presentations.

 

Thursday, October 3

10:15 - 10:30 a.m.

Booth #429, Exhibit Hall

 

Detecting MRSA Without Culture - A Path Towards Broader Antimicrobial Resistance Detection

Presented by Sivan Bercovici, PhD | Chief Technology Officer, Karius

Identifying antimicrobial resistance (AMR) promotes antimicrobial stewardship. Performing AMR identification directly from blood, using microbial cell-free DNA, offers a culture-independent method while potentially decreasing turnaround time for results. Here we present the analytical validation of the Karius Test for methicillin-resistant and -sensitive Staphylococcus aureus (MRSA and MSSA) detection. The presentation will outline the validation process, discussing key elements that are relevant to detecting an expanding portfolio of AMR mechanisms.


Friday, October 4

12:00 - 12:15 p.m.

Booth #429, Exhibit Hall

 

Identification of Bartonella Species in Culture-Negative Endocarditis Using the Karius Test

Presented by Susan Russo, RN, CPNP, CPHON | Pediatric Infectious Disease Nurse Practitioner, Dell Children's Hospital

Infective endocarditis can be life-threatening and up to 55% of cases are culture negative1. Identification of the causal pathogen is critical to improving outcomes. Here we present two cases of culture-negative endocarditis (CNE) where the Karius Test accurately identified Bartonella henselae and Bartonella vinsonii and enabled rapid and targeted antimicrobial treatment. In both patients, serial sampling demonstrated a predictable response in the microbial cell-free DNA (mcfDNA) level to antibiotic treatment and surgical resection. Plasma next-generation sequencing for mcfDNA offers a non-invasive means of identifying a spectrum of Bartonella species implicated in CNE. Furthermore, the quantitative aspect of the test may offer a means to monitor response to therapy.

1 Tattevin P. et al Med Mal Infect 2015; 45:1–8.


Saturday, October 5

10:15 - 10:30 a.m.

Booth #429, Exhibit Hall

 

The Karius Test Reveals a Wide Diversity of Legionella and Nocardia Species in Clinical Infections

Presented by Asim Ahmed, MD | Senior Medical Director, Karius

Legionella and Nocardia can cause diagnostically challenging infections. The diagnosis of Nocardia often requires invasive sampling and direct culture of infected tissue (lung, brain or skin). The urine Legionella antigen test only detects L. pneumophila serogroup 1. The Karius Test can detect over 1,000 pathogens including more than 30 species of Legionella and over 70 species of Nocardia from a single blood sample. We present a series of 40 patients in whom the Karius Test identified a diverse array of Legionella and Nocardia species causing infection including one patient with a co-infection of both. The Legionella detections comprise a number of species beyond L. pneumophila. Accurate species level identification of Legionella has important public health implications given the pathogen’s association with environmental, waterborne outbreaks. Most of the Nocardia detections belonged to the Nocardia asteroides complex. Remarkably in four cases the Nocardia detection demonstrated read sharing among closely related species and likely represent potentially novel strains. Next-generation sequencing of microbial cell-free DNA offers a rapid, non-invasive, culture-independent means of diagnosing Legionella and Nocardia infections, capturing species diversity and uncovering new insights on genus complexity not resolved by current taxonomic classification.

 

 

From a single blood sample, 1,000+ pathogens can be identified with the Karius Test. With this comes knowledge, clarity, and confidence.

 

Schedule a meeting with our Medical Team during IDWeekTM or post-conference.

 

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Abstracts

Thursday, October 3

12:15 – 1:30 p.m.
Poster Presentations
Poster Hall

Saturday, October 5

12:15 – 1:30 p.m.
Poster Presentations
Poster Hall